Integrating experimental and computational methods to uncover the anti-inflammatory, anti-nociceptive and antioxidant properties of Alchemilla vulgaris
- By H. Saiah,
- W. Saiah,
- F. Nehal,
- M. Mokhtar
- and T. Aburjai
Pages 274 to 294
Cite this article
- SAIAH, H.,
- SAIAH, W.,
- NEHAL, F.,
- MOKHTAR, M.
- and ABURJAI, T.,
- Saiah, H..,
- et al.
- Saiah, H.,
- Saiah, W.,
- Nehal, F.,
- Mokhtar, M.
- and Aburjai, T.
https://doi.org/10.3166/phyto-2024-0421
Cite this article
- Saiah, H.,
- Saiah, W.,
- Nehal, F.,
- Mokhtar, M.
- and Aburjai, T.
- Saiah, H..,
- et al.
- SAIAH, H.,
- SAIAH, W.,
- NEHAL, F.,
- MOKHTAR, M.
- and ABURJAI, T.,
https://doi.org/10.3166/phyto-2024-0421
The development of novel cyclooxygenase-2 inhibitors is critical for patients suffering from chronic pain and inflammatory disorders. Herein, we report on the therapeutic activity, molecular docking, and pharmacokinetic parameters (ADMET) of Alchemilla vulgaris extract. Anti-inflammatory activity was assessed using a carrageenan-induced rat paw edema test. An acetic acid-induced pain modulation assay was performed to study analgesic activity. In vitro tests were conducted to determine scavenging activity against DPPH and inhibition of lipid peroxidation. Molecular docking of A. vulgaris major compounds was investigated against COX-2. Our findings demonstrated that the extract contained an important amount of phenolic compounds. The extract’s antioxidant activity was demonstrated by IC50 values of 808.49 μg/mL and 1357.35 μg/mL against DPPH radical and lipid peroxidation, respectively. Furthermore, the in vivo anti-inflammatory assay demonstrated the maximum (11.46%) reduction of edema at 400 mg/kg b.w. Furthermore, a significant (p < 0.001) pain modulation activity was revealed. These in vivo findings were corroborated by molecular docking investigations; the binding energies of A. vulgaris phytoconstituents were found in the range of – 6.5 to – 9.8 kcal/mol. The obtained results revealed that the antioxidant, anti-inflammatory, and analgesic activities of A. vulgaris extract were in consensus with the ADMET predictions.
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